DEVELOPMENT OF NEW BIODEGRADABLE COMPOSITIONS OF WOUND COVERINGS AND THE STUDY OF THEIR REGENERATIVE PROPERTIES

Abstract

The compositions of wound coatings based on collagen (WCC) have been created. They were isolated from cattle tendons with additives of biologically active compounds, such as alkaloids from plants of the genus Convolvulus (convolvin, convolamin, phyllalbine), and flavonoids isolated from the buds of the Saphora japonica plant (quercetin, rutin). The study showed that alkaloids and flavonoid rutin have antimicrobial activity. The WCC with alkaloids were rejected based on the physical-chemical properties, due to low adhesion and strength. The WCC with quercetin (QU-WCC), phyllalbine (F-WCC) and rutin (RU-WCC) showed a good effect in the treatment of purulent wounds of the skin. In experimental models of purulent wounds, QU-WCC reduced the time of epithelialization of purulent wounds by 11.1-12.2 days (fully wounds were epithelized by 17.2 ± 0.1 days), RUWCC reduced the terms of epithelialization of purulent wounds by 8.4 – 9.2 days (fully wounds were epithelized for 20.1 ± 0.2 days), F-WCC reduced the time of epithelialization of purulent wounds by 11,5-12,4 days (fully wounds were epithelized by 16,9±0,2 days) compared with control wounds with no treatment (completely wounds were epithelized for 28.9 ± 0.6 days). The effect is due to the antioxidant properties of quercetin and rutin, as well as the antimicrobial activity of rutin and the natural, hypoallergenic and histocompatible collagen matrix. The obtained RP compositions are non-toxic (hazard class 5), do not cause local irritation, are histocompatible, hypoallergenic and correspond to the properties specified for wound coverings.